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Summary - (CDC) Biosafety in Microbiological and Biological Labs

Under CDC guidelines, for work at biosafety level 2 and above, the Principal Investigator must:

  1. limit or restrict access to the laboratory when work with infectious agents is in progress, including determining who may be at increased risk;
  2. establish policies and procedures whereby only persons who have been advised of the potential hazards and meet specific entry requirements (e.g., immunization) may enter the laboratory;
  3. ensure that laboratory personnel are offered appropriate immunizations or tests for the agents handled or potentially present in the laboratory (e.g., hepatitis B vaccine or TB skin testing);
  4. select and provide appropriate personal protective equipment required for work with hazardous materials;
  5. ensure that the biohazard symbol is used to identify equipment, containers, rooms and materials that contain or are contaminated with viable hazardous agents or human blood/tissue;
  6. ensure that a biological safety cabinet is available for procedures with a potential for creating infectious aerosols or splashes (centrifuging, grinding, blending, vigorous shaking or mixing, sonic disruption, opening containers with different internal pressures, inoculating animals intranasally, harvesting infected tissues from animals or embryonate eggs), or high concentrations or large volumes of infectious agents or organisms containing recombinant DNA molecules are used.
  7. ensure that laboratory and support personnel receive appropriate training on the potential hazards associated with the work involved, the necessary precautions to prevent exposures, and the exposure evaluation procedures, and that personnel receive annual updates or additional training as necessary for procedural or policy changes; and
  8. develop standard operating procedures incorporating biosafety procedures or a biosafety manual prepared specifically for the laboratory and safety procedures for toxins, advise personnel of special hazards, and require them to read and follow instructions on practices and procedures (see BSL-2 SOPs and Animal BSL-2 SOPs for help).

Institutional Biosafety Committee (IBC)

BMSB 209


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