Step 2 Copy the pure mRNA into a DNA molecule (cDNA = complementary DNA) using an enzyme called "reverse transcriptase" and the precursors of DNA called nucleotides.

Diagram

Step 3

Insert the cDNAs into a plasmid, a circular replicating DNA element, so that you can make move the gene into a bacterium (this step involves use of restriction enzymes [to cut] and ligase [to paste]. The insert is placed behind a transcriptional promoter to make more RNA.

Diagram
Step 1

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Introduction
Step 1
Steps 2 & 3
Steps 4 & 5
Steps 6, 7, & 8
Steps 4 & 5


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