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| 1. | Identify any specific disease state for the 100,000 genes |
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Targets |
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a. | valid therapeutic target molecule |
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b. | related molecules which may cross-react |
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c. | transporters & metabolizing enzymes which may interact with target molecule |
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d. | genetic variation in patient population that would impact therapeutic efficacy or toxicity |
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Methodology |
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a. | identify & characterize targets - find a small molecule which interacts with target |
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b. | sequence genes/proteins related to target - isozymes, isoforms |
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c. | find & sequence other genes in the pathway or pharmacologically-related - ADME - related genes (adsorption, distribution, metabolism, & excretion) |
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d. | find genes / alleles that contribute to susceptibility, rate of progression / severity in individual patients |
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| 2. | Expression profiling |
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Target - cDNA or oligonucleotide arrays in various states of: |
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a. | diseases (see if missing or too much of one mRNA) |
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b. | tissues (e.g. specific for heart or brain?) |
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c. | tumor (gene gone bad or making too much?) |
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Methodology |
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a. | select candidate gene that is involved in process |
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b. | identify different alleleic forms in a population |
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c. | genotype individuals with disease to see most common allele |
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d. |
assess if:
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e. | screening / monitoring or prophylactic intervention |
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Return to College Education and Training |
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