Graduate Student Seminar: "Modulation of oxidative stability of hemoglobin using ascorbate and riboflavin." Presented by Chanpreet Juneja, graduate student, Dept. of Pharmaceutical Sciences, OU College of Pharmacy (Graduate advisor: Dr. Vibhudutta Awasthi)
Seminar is from noon-1 pm, CPB 101 (OKC) and 1D29 (Tulsa)
Hemoglobin is the protein responsible for oxygen transport in our body. Susceptibility of hemoglobin to oxidation is a critical limitation in formulation of liposome encapsulated hemoglobin (LEH). Upon oxidation, hemoglobin changes to oxidized counterpart methemoglobin, which is incapable of carrying oxygen. The goals of our research are 1) To prevent the oxidation of hemoglobin to methemoglobin 2) To reclaim hemoglobin from methemoglobin.
It has been shown that ascorbate is capable of reducing methemoglobin. Our preliminary studies have indicated that an optimized combination of ascorbate and riboflavin is more effective than ascorbate in preventing the oxidation of hemoglobin as well as reclaiming the hemoglobin from its oxidized form. These results can have a significant impact on the process of isolation of hemoglobin from outdated erythrocytes and formulation of liposome encapsulated hemoglobin. The outcomes of this research could possibly play a significant role in improving the storage life of LEH, a potential candidate for effective blood substitute.