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Yusuke Takahashi, PhD
Associate Professor of Research

Mailing Address:
941 Stanton L. Young Blvd., BSEB 331A
Oklahoma City, OK 73104-5020

(405) 271-5896 ext 48493
(405) 271-7522


Research Interests

  1. Regulatory role of microRNAs in Diabetes.
  2. The retinoid cycle in vertebrate vision. Particularly RPE65, a key enzyme (isomerohydrolase) in the visual cycle.
  3. Molecular basis of spectral tuning in visual pigments and adaptive evolution of color vision.



1997-2000 PhD - Information Science, Tohoku University, Miyagi, Japan


Recent Publications

Anne R. Murray, Qian Chen, Yusuke Takahashi*, Kevin K. Zhou, Kyoungmin Park and Jian-xing Ma., MicroRNA-200b downregulates oxidation resistance 1 (Oxr1) expression in the retina of type 1 diabetes model., Invest. Ophtalmol. Vis. Sci., 2013, 54, 1689-1697, (* corresponding author).

Yusuke Takahashi, Gennadiy Moiseyev, Olga Nikolaeva and Jian-xing Ma., Identification of the key residues determining the product specificity of isomerohydrolase., Biochemistry, 2012, 51, 4217-4225.

Kyungwon Lee, Yang Hu, Lexi Ding, Ying Chen, Yusuke Takahashi, Robert Mott, Jian-xing Ma., Therapeutic Potential of a Monoclonal Antibody Blocking the Wnt Pathway in Diabetic Retinopathy., Diabetes, 2012, 61, 2948-2957.

Ti Zhou, Xuemin He, Rui Cheng, Bing Zhang, R. R. Zhang, Ying Chen, Yusuke Takahashi, Anne R. Murray, Kyungwon Lee, G. Gao and Jian-xing Ma., Implication of dysregulation of the canonical wingless-type MMTV integration site (WNT) pathway in diabetic nephropathy., Diabetologia, 2012, 55, 255–266.

Yusuke Takahashi, Gennadiy Moiseyev, Ying Chen, Olga Nikolaeva, and Jian-xing Ma., An alternative isomerohydrolase in the retinal Müller cells of a cone-dominant species., FEBS J, 2011, 278, 2913-2926.

Yusuke Takahashi, Gennadiy Moiseyev, Ying Chen, Olga Nikolaeva, Krysten Farjo,and Jian-xing Ma., An Enzymatic Mechanism for Generating the Precursor of Endogenous 13-cis Retinoic Acid in the Brain., FEBS J, 2011, 278, 973-987. PMCID: 3073448

Yang Hu, Ying Chen, Gennadiy Moiseyev, Yusuke Takahashi, Robert Mott, Jian-xing Ma. Comparison of ocular pathologies in vitamin A-deficient mice and RPE65 gene knockout mice., Invest. Ophtalmol. Vis. Sci., 2011, 52, 5507-5514. PMCID: PMC3176065.

Gennadiy Moiseyev, Olga Nikolaeva, Ying Chen, Krysten Farjo, Yusuke Takahashi and Jian-xing Ma., Inhibition of the visual cycle by A2E through direct interaction with retinal pigment epithelium (RPE) 65 and implications in Stargardt disease., Proc. Nat. Acad. Sci. U.S.A., 2010, 107, 17551-17556. PMCID: PMC2955102.

Nikolaeva O, Takahashi Y, Moiseyev G, and Ma J-x. Negative charge of the glutamic acid 417 residue is crucial for isomerohydrolase activity of RPE65., Biochem Biophys Res Commun, 2010, 391, 1757-1761.

Nikolaeva O, Takahashi Y, Moiseyev G, and Ma J-x. Purified RPE65 shows isomerohydrolase activity after reassociation with a phospholipid membrane., FEBS J, 2009, 276, 3020-3030.

Farjo K, Moiseyev G, Takahashi Y, Crouch RK, and Ma J-x. RDH10 has 11-cis-Retinol Dehydrogenase Activity and Interacts with Visual Cycle Proteins., Invest Ophtalmol Vis Sci, 2009, 50, 5089-5097.

Takahashi Y, Moiseyev G, Farjo K, and Ma J-x. Characterization of key residues and membrane association domains in RDH10., Biochem J, 2009, 419, 113-122.

Takahashi Y, Moiseyev G, Ablonczy Z, Chen Y, Crouch RK, and Ma J-x. Identification of a Novel Palmitylation Site Essential for Membrane Association and Isomerohydrolase Activity of RPE65., J Biol Chem, 2009, 284, 3211-3218

Moiseyev G, Takahashi Y, Chen Y, Kim S, and Ma, J-x. RPE65 from Cone-dominant Chicken Is a More Efficient Isomerohydrolase Compared with That from Rod-dominant Species. J Biol Chem 283: 8110-7, 2008.

Takahashi Y, Moiseyev G, Chen Y, and Ma J-x. The roles of three palmitoylation sites of RPE65 in its membrane association and isomerohydrolase activity. Invest Ophtalmol Vis Sci, 47:5191-5196, 2006.

Chen Y, Moiseyev G, Takahashi Y, and Ma J-x. Impacts of two point mutations of RPE65 from Leber's congenital amaurosis on the stability, subcellular localization and isomerohydrolase activity of RPE65. FEBS Lett., 580:4200-4204, 2006.

Takahashi Y, Chen Y, Moiseyev G, and Ma J-x. Two point mutations of RPE65 from patients with retinal dystrophies decrease the stability of RPE65 protein and abolish its isomerohydrolase activity. J Biol Chem, 281:21820-21826, 2006.

Chen Y, Moiseyev G, Takahashi Y, and Ma J-x. RPE65 Gene Delivery Restores Isomerohydrolase Activity and Prevents Early Cone Loss in Rpe65-/- Mice. Invest Ophtalmol Vis Sci, 47:1177-1184, 2006.

Moiseyev G, Takahashi Y, Chen Y, Gentleman S, Redmond TM, Crouch RK, and Ma J-x. RPE65 is an Iron(II)-dependent isomerohydrolase in the retinoid visual cycle. J Biol Chem, 281:2835-2840, 2006.

Takahashi Y, Moiseyev G, Chen Y, and Ma J-x. Identification of conserved histidines and glutamic acid as key residues for isomerohydrolase activity of RPE65, an enzyme of the visual cycle in the retinal pigment epithelium. FEBS Lett., 579:5414-5418, 2005.

Moiseyev G, Chen Y, Takahashi Y, Wu B, and Ma J-x. RPE65 is the isomerohydrolase in the visual cycle. Proceedings of the National Academy of Science U.S.A., 102:12413-12418, 2005.

Takahashi Y, and Yokoyama S. Genetic basis of spectral tuning in the violet-sensitive visual pigment of African clawed frog. Xenopus laevis., Genetics, 171:1153-1160, 2005.

Yokoyama S, Starmer WT, Takahashi Y, and Tada T. Tertiary structure and spectral tuning of UV and violet pigments in vertebrates. Gene, 365:95-103, 2005.

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The University of Oklahoma Health Sciences Center

Endocrinology and Diabetes
1000 N. Lincoln Blvd.; Harold Hamm Diabetes Center Building #2900
Oklahoma City, OK 73104
Phone - (405) 271-5896
FAX - (405) 271-7522

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