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The University of Oklahoma

Police Department

SPILLS ON THE BODY

  • Remove contaminated clothing.
  • Vigorously wash exposed area with soap and water for one minute.
  • Obtain medical attention (if necessary).
  • Report the incident to the laboratory supervisor.

BIO SAFETY LEVEL 1 ORGANISM SPILL

  • Wear disposable gloves.
  • Soak paper towels in disinfectant and place over the spill.
  • Place towels in a plastic bag for disposal.
  • Clean up spill area with fresh towels soaked in disinfectant.

BIO SAFETY LEVEL 2 ORGANISM SPILL (moderate risk agents)

  • Alert people in the immediate area of the spill.
  • Put on protective equipment. This may include a laboratory coat with long sleeves, back-fastening gown or jumpsuit, disposable gloves, disposable shoe covers, safety goggles, mask or full-face shield.
  • Cover the spill with paper towels or other absorbent materials.
  • Carefully pour a freshly prepared 1 to 10 dilution of household bleach around the edges of the spill and then into the spill. Avoid splashing.
  • Allow a 20-minute contact period.
  • After the spill has been absorbed, clean up the spill area with fresh towels soaked in disinfectant.
  • Place towels in a plastic bag and decontaminate in an autoclave.

BIO SAFETY LEVEL 3 ORGANISM SPILL

  • Do not breath; leave the room immediately and close the door.
  • Notify others in the room to evacuate immediately, and assist others if necessary.
  • Remove personal protective equipment in the airlock or access zone, turn potentially contaminated clothing outward, remove gloves last, and wash any exposed skin areas with antiseptic soap and warm water.
  • Call the Campus Police and Public Safety Department at 1+4911 and alert them of the situation.
  • Warn others not to enter the contaminated area. Place an appropriate sign on the door.
  • Wait at least 30 minutes to allow dissipation of aerosols created by the spill.
  • Put on a long sleeved gown, gloves, appropriate respirator, and rubber boots, if required, before re-entering the room.
  • Cover the spilled area with paper towels or disinfectant soaked paper towels.
  • Slowly pour appropriate decontaminant solution around the spill and allow to flow into the spill. Avoid splashing or the creation of aerosols during this step.
  • Let stand at least 15 - 20 minutes to allow adequate contact time.
  • Using an autoclavable dust pan and squeegee, transfer all contaminated materials (paper towels, glass, liquid, gloves, etc.) into a deep autoclave pan, and autoclave promptly.
  • Repeat the decontamination procedures.
  • The dust pan and squeegee should be placed in an autoclave bag and autoclaved as well.